Service-Telefon
+49(0)6151-988520
German English
Warenkorb      SPACERIMGALTERNATEanzeigen
Artikelanzahl:  1
Warenwert:
Versandkosten:
Gesamtbetrag:
Fachgebiete

Details

2187577 Helmchen, Fritjof; Konnerth, Arthur:
Imaging in Neuroscience: A Laboratory Manual
   Preis:   € 209,50

Einband: Hardcover
Verlag: Cold Spring Harbor Laboratory Press
Erscheinungsdatum: 05/2011
Seiten: 736 S.
Abbildungen: illus.index

ISBN-10: 0-87969-937-X   
ISBN-13: 9780-87969-937-6

In den Warenkorb

Weitere Fachbücher aus dem Fachgebiet:
Laborhandbücher | Mikroskopie | Neurobiologie | Zellbiologie | 
zurück
Beschreibung
Neuroscientists have often pioneered the use of new visualization techniques.  Imaging in Neuroscience: A Laboratory Manual provides the definitive collection of methods in use in this groundbreaking field. With more than 90 chapters, the manual offers a depth of coverage unavailable from any other source. Sections focus on imaging at the molecular level, axons and nerve terminals, spines and dendrites, neurons and circuits in vitro, neurons and circuits in vivo, glia, brain dynamics and behavior, and brain pathology. Protocols range from basic techniques to recent breakthroughs. Imaging in Neuroscience: A Laboratory Manual is an essential guide to discovering and implementing these techniques.
Inhalt
(preliminary)
Preface to the Book Series
Preface to Book 2

SECTION 1: MOLECULAR TOOLS FOR IMAGING
1. Tracking Receptors Using Individual Fluorescent and Nonfluorescent Nanolabels
2. Imaging Single Receptors with Quantum Dots
3. Synapto-pHluorins: Genetically Encoded Reporters of Synaptic Transmission
4. Spatially Resolved Flash Photolysis via Chemical Two-Photon Uncaging
5. Engineering Light-Regulated Ion Channels
6. Microbial Opsins: A Family of Single-Component Tools for Optical Control of Neural Activity
7. Measuring Membrane Voltage with Fluorescent Proteins
8. Imaging Neuronal Activity with Genetically Encoded Calcium Indicators
9. Imaging Synaptic Inhibition with Clomeleon, a Genetically Encoded Chloride Indicator
10. Recombinant Fluorescent Rabies Virus Vectors for Tracing Neurons and Synaptic Connections
11. Generating and Imaging Multicolor Brainbow Mice
12. The Use of BAC Transgenic Mice to Label Genetically Defined Cell Populations and the GENSAT Database of Engineered Mouse Strains

SECTION 2: AXONS AND NERVE TERMINALS
13. Presynaptic Calcium Measurements Using Bulk Loading of Acetoxymethyl Indicators
14. Imaging Presynaptic Calcium Transients Using Dextran-Conjugated Indicators
15. Confocal Spot Detection of Presynaptic Ca2+ Domains
16. Ca2+ Uncaging in Nerve Terminals
17. Multiple Light Scattering Changes Associated with Secretion from Peptidergic
18. Imaging Synaptic Vesicle Recycling Using FM Dyes
19. Imaging Exocytosis with Total Internal Reflection Microscopy
20. Interferometric Detection of Action Potentials
21. Imaging Sodium in Axons and Dendrites
22. Generation and Screening of Mice with Transgenic Neuronal Labeling Controlled by thy1-Regulatory Elements

SECTION 3: SPINES AND DENDRITES
23. Correlated Light and Electron Microscopy of GFPLabeled Dendrites and Axons
24. Stimulated Emission Depletion (STED) Imaging Dendritic Spines
25. Two-Photon Uncaging Microscopy
26. Acousto-Optical Delector–Based Patterned Ultraviolet-Uncaging of Neurotransmitter for the Study of Neuronal Integration
27. Two-Photon Calcium Imaging of Dentritic Spines
28. Imaging Calcium Waves and Puffs in Central Neurons
29. Dendritic Voltage Imaging
30. Two-Photon Sodium Imaging in Dendritic Spines
31. Transcranial Two-Photon Imaging of the Living Mouse Brain
32. Imaging Neocortical Neurons Through a Chronic Cranial Window

SECTION 4: NEURONS AND CIRCUITS IN VITRO
33. Maintaining Live Cells and Tissue Slices in the Imaging Setup
34. Calcium Imaging in Neuronal Endoplasmic Reticulum
35. Dye Loading with Whole-Cell Recordings
36. A Single-Compartment Model of Calcium Dynamics
37. Imaging Action Potentials with Calcium Indicators
38. A Practical Guide to Studying Genetically Defined Neurons
39. Visually Guided Neurotransmitter Uncaging on Dendrites
40. Uncaging Calcium in Neurons
41. An Optical Fiber-Based Uncaging System
42. Multiphoton Stimulation of Neurons and Spines
43. Circuit Mapping by Ultraviolet Uncaging of Glutamate
44. Circuit Mapping with Two-Photon Uncaging
45. All-Optical, In Situ Histology of Brain Tissue with Femtosecond Laser Pulses
46. Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System

SECTION 5: NEURONS AND CIRCUITS IN VIVO
47. Optically Guided In Vivo Recordings
48. Imaging with Voltage-Sensitive Dyes: Spike Signals, Population Signals, and Retrograde Transport
49. In Vivo Two-Photon Calcium Imaging Using Multicell Bolus Loading of Fluorescent Indicators
50. In Vivo Local Electroporation Dye Loading for Ca2+ Imaging and Neuronal-Circuit Tracing
51. Two-Photon Calcium in the Visual Cortex
52. Fast Three-Dimensional Population Imaging
53. High-Speed Population Calcium Imaging Using Acousto-Optic Scanners
54. Calcium Imaging in the Drosophila Olfactory System with a Genetic Indicator
55. In Vivo Calcium Imaging in the Zebrafish and Mouse Olfactory System
56. Calcium Imaging in Populations of Olfactory Neurons by Planar Illumination Microscopy
57. Functional Neuron-Specific Expressions of Genetically-Encoded Fluorescent Calcium Indicator Proteins in Living Mice
58. Chronic Calcium Imaging of Neocortical Neurons Using a Troponin C-Based Indicator
59. Two-Photon Chloride Imaging Using MQAE In Vitro and In Vivo
60. Intrinsic Optical Imaging of Functional Map Development in Mammalian Visual Cortex

SECTION 6: GLIA
61. Imaging Astrocytic Calcium and Vacuole-Like Vesicles
62. Monitoring Exocytosis in Astrocytes with Total Internal Reflection Fluorescence Microscopy
63. In Vivo Labeling of Astroglia with Sulforhodamine 101
64. In Vivo Imaging of Astroglia Dynamics
65. Imaging Calcium Waves in Cerebellar Bergmann Glia
66. In Vivo Calcium Imaging of Cerebellar Glia with Synthetic and Genetic Indicators
67. Two-Photon Imaging of Neurons and Glia in the Spinal Cord In Vivo
68. Imaging Microglia in Brain Slices and Slice Cultures
69. Two-Photon Imaging of Cerebellar Astrocytic and Neuronal Activity in Awake Mobile Mice

SECTION 7: BRAIN DYNAMICS AND BEHAVIOR
70. Imaging Caenorhabditis elegans behavior
71. In Vivo Dendritic Calcium Imaging in the Fly Visual System
72. Imaging Neuronal Activity and Motor Behavior in Zebrafish
73. Confocal Calcium Imaging of Neuronal Activity in Larval Zebrafish
74. Voltage-Sensing Dyes
75. Voltage-Sensitive Dye Imaging of Cortical Spatiotemporal Dynamics in Awake Behaving Mice
76. Two-Photon Imaging of Neural Activity in Awake Mobile Mice
77. Imaging Activity from Neuronal Populations in the Awake and Anesthetized Rodent
78. Two-Photon Fiberscope
79. Optogenetics: Opsins and Optical Interfaces in Neuroscience
80. Optogenetics in Freely Moving Mammals: Dopamine and Reward
81. In Vivo Calcium Recordings and ChR-2 Activation through a Single Optical Fiber
82. Fiber-Optic Calcium Monitoring of Dendritic Activity
83. Imaging the Neocortex Functional Architecture Based on Multiple Intrinsic Signals: Implications for Hemodynamic Based Functional Imaging

SECTION 8: BRAIN PATHOLOGY
84. Two-Photon Imaging of Blood Flow in Cortex
85. Optical-Based Models of a Single Blood Vessel Occlusion in Rodent Neocortex
86. Two-Photon Imaging of Neuronal Structural Plasticity in Mice during and after Ischemia
87. Two-Photon Imaging of Microglia Cells in the Mouse Brain In Vivo
88. Two-Photon Imaging of Immune Cells in Neural Tissue
89. Two-Photon Imaging of Structure and Function in Alzheimer’s Disease
90. Two-Photon Imaging of Neural Networks in a Mouse Model of Alzheimer’s Disease
91. Imaging Tumors in the Brain
92. Fluorescent Molecular Tomography of Brain Tumors in Mice

SECTION 9: APPENDICES
1. Electromagnetic Spectrum
2. Fluorescence Microscopy Filters and Excitation/Emission Spectra
3. Safe Operation of a Fluorescence Microscope
4. Microscope Objective Lenses
5. Glossary
6. Cautions

Zielgruppe/Leser
Zellbiologie
Mikroskopie
Neurobiologie
Laborhandbuch

Autoreninfo
Fritjof Helmchen, Brain Research Institute, University of Zurich, Switzerland;
Arthur Konnerth, Institute for Neurosciences, Technical University Munich, Germany
 © academic-books.com, Alle Rechte vorbehalten.