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2044542 Papkovsky, Dmitri B.:
Live Cell Imaging
Methods and Protocols
Preis:   € 96,25

Band: 591
Auflage: 1st Edition.
Verlag: Humana Press
Erscheinungsdatum: 12/2009
Seiten: 367 S.

ISBN-10: 1-60761-403-0   
ISBN-13: 978-1-60761-403-6


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Beschreibung
Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.
Inhalt
Part I: General Principles and Overview



1. Instrumentation for Live-Cell Imaging and Main Formats

Martin Oheim



2. Labels and Probes for Live Cell Imaging: Overview and Selection Guide

Scott Hilderbrand



3. Live Cell Imaging: An Industrial Perspective

Terry McCann





Part II: Imaging Techniques, Probes, and Applications



4. Design of Fluorescent Fusion Protein Probes

Elizabeth Pham and Kevin Truong



5. Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells

Dan Yang, Zhen-Ning Sun, Tao Peng, Hua-Li Wang, Jian-Gang Shen, Yan Chen, and Paul Kwong-Hang Tam



6. Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells

Wen-hong Li



7. The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosis

Ronald S. Flannagan and Sergio Grinstein



8. Imaging of Mitotic Cell Division and Apoptotic Intra-Nuclear Processes in Multicolor

Kenji Sugimoto and Shigenobu Tone



9. Manipulation of Neutrophil-Like HL-60 Cells for the Study of Directed Cell Migration

Arthur Millius and Orion D. Weiner



10. A Method for Analyzing Protein-Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy

Hae Won Sohn, Pavel Tolar, Joseph Brzostowski, and Susan K. Pierce



11. Sample Preparation for STED Microscopy

Christian A. Wurm, Daniel Neumann, Roman Schmidt, Alexander Egner, and Stefan Jakobs



12. Two-Photon Permeabilization and Calcium Measurements in Cellular Organelles

Oleg Gerasimenko and Julia Gerasimenko



13. Imaging and Analysis of Three-Dimensional Cell Culture Models

Benedikt W. Graf and Stephen A. Boppart



14. Long-Term Imaging in Microfluidic Devices
Gilles Charvin, Catherine Oikonomou, and Frederick Cross



15. Monitoring of Cellular Responses to Hypoxia

Christoph Wotzlaw and Joachim Fandrey



16. Imaging of Cellular Oxygen and Analysis of Metabolic Responses of Mammalian Cells

Andreas Fercher, Tomas C. O?Riordan, Alexander V. Zhdanov, Ruslan I. Dmitriev, and Dmitri B. Papkovsky



17. Analysis of Mitochondrial pH and Ion Concentrations

Martin vandeVen, Corina Balut, Szilvia Baron, Ilse Smets, Paul Steels, and Marcel Ameloot



18. Live Cell Imaging Analysis of Receptor Function

Daniel C. Worth and Maddy Parsons



19. Subcellular Dynamic Imaging of Protein-Protein Interactions in Live Cells by Bioluminescence Resonance Energy Transfer

Julie Perroy



20. Quantitative Analysis of Membrane Potentials

Manus W. Ward



21. Image Correlation Spectroscopy to Define Membrane Dynamics

Jeremy Bonor and Anja Nohe
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