Inhalt Strategies of Protein Purification & Characterization.
Computational Analysis.
Detection and Assay Methods.
Extraction, Stabilization, and Concentration.
Production of Recombinant Proteins.
Purification of Recombinant Proteins.
Characterization of Recombinant Proteins.
Conventional Chromatographic Separations.
Affinity Purification.
Electrophoresis-Updates Expected for CPPS 10.1, 10.3, 10.10 & 10.11.
Chemical Analysis.
Post-Translational Modification: Glycosylation.
Post-Translational Modification: Phosphorylation and Phosphatases.
Post-Translational Modification: Specialized Applications.
Chemical Modification of Proteins.
Mass Spectrometry.
Preparation and Handling of Peptides.
Identification of Protein Interactions.
Quantitation of Protein Interactions.
Peptidases.
Appendix 1: Reagents and Solutions.
Appendix 2: Useful Measurements.
Appendix 3: Commonly Used Techinques.
Appendix 4: Suppliers.
References.
Zielgruppe/Leser Biowissenschaftler
Autoreninfo Dunn, B. M. / Speicher, D. W. / Wingfield, P. T.
B. M. Dunn, University of Florida, Gainesville, Fla.; D. W. Speicher, The Wistar Institute, Philadelphia, Penn.; P. T. Wingfield, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Md
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