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2041494 Doering, Laurie C.:
Protocols for Neural Cell Culture
  Preis:   € 80,20

Auflage: 4th ed.
Verlag: Humana Press
Erscheinungsdatum: 10/2009
Seiten: 385 S.

ISBN-10: 1-60761-291-7   
ISBN-13: 978-1-60761-291-9


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Beschreibung
Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results.
Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions? tradition of exceptional quality and thorough coverage in tissue culture protocols for today?s neuroscience in order to inspire researchers in the field to further explore this imperative area of study.
Inhalt
1. Neurosphere and Neural Colony Forming Cell Assays
Sharon A. Louis and Brent A. Reynolds

2. Directed Neuronal Differentiation of Embryonic and Adult Derived Neurosphere Cells
Marcos R. Costa, Ravi Jagasia, and Benedikt Berninger

3. Culture and Differentiation of Human Neural Stem Cells
Soojung Shin and Mohan Vemuri

4. Neural Differentiation of Human Embryonic Stem Cells
Mirella Dottori, Alice Pébay, and Martin F. Pera

5. Isolation and Culture of Primary Human CNS Neural Cells
Manon Blain, Veronique E. Miron, Caroline Lambert, Peter J. Darlington, Qiao-Ling Cui, Philippe Saikali, V. Wee Yong, and Jack P. Antel

6. Bioengineering Protocols for Neural Precursor Cell Expansion
Behnam A. Baghbaderani, Arindom Sen, Michael S. Kallos, and Leo A. Behie

7. Intracellular Calcium Assays in Dissociated Primary Cortical Neurons
Navjot Kaur, David V.Thompson, David Judd, David R. Piper, and Richard G. Del Mastro

8. Dissociated Hippocampal Cultures
Francine Nault and Paul De Koninck

9. Primary Sensory and Motor Neuron Cultures
Andrea M. Vincent and Eva L. Feldman

10. Retinal Cell and Tissue Culture
Francisco L.A.F. Gomes and Michel Cayouette

11. Preparation of Normal and Reactive Astrocyte Cultures
Jean de Vellis, Cristina A Ghiani, Ina B. Wanner, and Ruth Cole

12. Oligodendrocyte Progenitor Cell Culture
Akiko Nishiyama, Ryusuke Suzuki, Hao Zuo, and Xiaoqin Zhu

13. Isolation of Microglia Subpopulations
Makoto Sawada and Hiromi Suzuki

14. Microglia from Progenitor Cells in Mouse Neopallium
Sergey Fedoroff and Arleen Richardson

15. Primary Schwann Cell Cultures
Haesun A. Kim and Patrice Maurel

16. Primary Dissociated Astrocyte and Neuron Co-Culture
Shelley Jacobs and Laurie C. Doering

17. Cerebellar Slice Cultures
Josef P. Kapfhammer

18. Hippocampal Slice Cultures
Jesse E. Hanson, Adrienne L. Orr, Silvia Fernandez-Illescas, Ricardo A. Valenzuela, and Daniel V. Madison

19. Molecular Substrates for Growing Neurons in Culture
Saulius Satkauskas, Arnaud Muller, Morgane Roth, and Dominique Bagnard

20. Guidance and Outgrowth Assays for Embryonic Thalamic Axons
Alexandre Bonnin

21. Detection of Cell Death in Neuronal Cultures
Sean P. Cregan

22. Live Imaging of Neural Cell Functions
Sabine Bavamian, Eliana Scemes, and Paolo Meda

23. Tissue Culture Procedures and Tips
Arleen Richardson and Sergey Fedoroff
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