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2183047 Schatten, Heide:
Germ Cell Protocols
Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning
Preis:   € 139,09

Band: 254, Einband: Pb
Auflage: 1st ed. Softcover of orig. ed. 2004
Verlag: Humana Press
Erscheinungsdatum: 10/2010
Seiten: 428 S.

ISBN-10: 1-61737-457-1   
ISBN-13: 978-1-61737-457-9


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Beschreibung
For volume 2 alone:



A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.



For both volumes:



A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. The methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer.

Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.
Inhalt
Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos

Michele Boiani, James Kehler, and Hans R. Schöler



Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans

Monika A. Jedrusik and Ekkehard Schulze



cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development

Myriam Hemberger



PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells: Cloning Uniquely Expressed RNAs from Rare Cells

Thiagarajan Venkataraman, Elio Dancausse, and Mary Lou King



Differentiating Embryonic Stem Cells into Embryoid Bodies

Gisele Höpfl, Max Gassmann, and Isabelle Desbaillets



The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation

Edward J. Gallagher and Jim McWhir



Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos

Jayne M. Squirrell and John G. White



Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes

Stephen A. Stricker



A Method for Producing Cloned Pigs by Using Somatic Cells as Donors

Liangxue Lai and Randall S. Prather



Pronuclear Microinjection of Mouse Zygotes

Thomas Rülicke



Parthenogenesis and Nuclear Transfer in Rabbit Oocytes

Yukio Tsunoda and Yoko Kato



Nuclear Transfer in Cattle

Gottfried Brem and Manzoor A. Nowshari



In Vitro Fertilization and Embryo Transfer in Felids

Charles Earle Pope



Production of Chimeric Chickens

Mitsuru Naito and Takashi Kuwana



Production of Haploid and Diploid Androgenetic Zebrafish

Bruce P. Brandhorst and Graham E. Corley-Smith



Construction and Detection of Fluorescent, Germline Transgenic Zebrafish

Elwood Linney and Ava J. Udvadia



Production of Zebrafish Germline Chimeras from Cultured Cells

Lianchun Fan, Annette Alestrom, Peter Alestrom, and Paul Collodi



Studying Nuclear Reprogramming with Cell Hybrids

Steve Pells and Jim McWhir



Cytoplasmic Transfer in Mammalian Eggs and Embryos

Henry E. Malter



Conventional Technologies for Cryopreservation of Human Oocytes and Embryos

Michael J. Tucker, Sharon Shipley, and Juergen Liebermann



Vitrifying and Warming of Human Oocytes, Embryos, and Blastocysts: Vitrification Procedures as an Alternative to Conventional Cryopreservation

Juergen Liebermann, and Michael J. Tucker



Microfluidic Removal of Cumulus Cells from Mammalian Zygotes

Henry C. Zeringue and David J. Beebe



Mammalian Embryo Culture in a Microfluidic Device

Eric M. Walters, Sherrie G. Clark, David J. Beebe, and Matthew B. Wheeler



Application of Alginate Gels to the Study of Mammalian Development

Richard L. Gardner



Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptosis in Preimplantation Mammalian Embryos

Steven F. Mullen and John K. Critser



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