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1744524 Strange, Kevin:
C. elegans
Methods and Applications
Preis:   € 139,09

Band: 351, Einband: Gb
Auflage: 1. Aufl.
Verlag: Humana Press
Erscheinungsdatum: 2006
Seiten: 308 S.

ISBN-10: 1-58829-597-4   
ISBN-13: 978-1-58829-597-2


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Beschreibung
Nonmammalian model organisms have become a cornerstone of systems biology research. Like the Rosetta Stone, which enabled modern scholars to decode ancient Egyptian hieroglyphics, model organisms enable biologists to decipher the genetic code underlying the complex physiological processes common to all life. C. elegans provides a particularly striking example of the experimental utility of model organisms. Genetic, molecular and systems biological characterization of this organism is greatly facilitated by its short life cycle and high rate reproduction, and by the ease with which it can be cultured in the laboratory. C. elegans also has a fully sequenced and well-annotated genome, which is assembled in readily accessible public databases along with virtually all other biological data on this organism.



C. elegans: Methods and Applications aims to enhance the readily available methodologies for the study of C. elegans by providing overviews and concise, step-by-step descriptions of the most state-of-the-art methods currently being utilized in the field. Some of the methods presented include forward and reverse genetic analysis, data mining and comparative genomics strategies, electron and fluorescence microscopy methods, automated imaging methods for worm behavioral analysis, functional genomics strategies, and cutting-edge methods for physiological analyses. This comprehensive collection of methods, written by experts in the field, endeavors to serve as a roadmap for researchers, illustrating the type or research that is possible with C. elegans, and illuminating its potential as a vehicle for future discovery.
Inhalt
An Overview of C. elegans Biology

Kevin Strange



Comparative Genomics in C. elegans, C. briggsae and Other Caenorhabditis Species

Avril Coghlan, Jason E. Stajich and Todd W. Harris



WormBase: Methods for Data Mining and Comparative Genomics

Todd W. Harris and Lincoln D. Stein



C. elegans Deletion Mutant Screening

Robert J. Barstead and Donald G. Moerman



Insertional Mutagenesis in Caenorhabditis elegans Using the Drosophila Transposon Mos1: A Method for the Rapid Identification of Mutated Genes

Jean-Louis Bessereau



SNP Mapping

M. Wayne Davis and Marc Hammarlund



Creation of Transgenic Lines Using Microparticle Bombardment Methods

Vida Praitis



Construction of Plasmids for RNAi and In Vitro Transcription of dsRNA

Lisa Timmons



Delivery Methods for RNAi in Caenorhabditis elegans

Lisa Timmons



Functional Genomic Approaches in C. elegans

Todd Lamitina



Assays for Toxicity Studies in C. elegans with Bt Crystal Proteins

Larry J. Bischof, Danielle L. Huffman and Raffi V. Aroian



Fluorescent Reporter Methods

Harald Hutter



Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans

Michael M. Francis and Andres Villu Maricq



Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis

Michael A. Miller



Preservation of C. elegans Tissue via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immuno-Cytochemistry

Robby M. Weimer



Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants

Keith Nehrke



Automated Imaging of C. elegans Behavior

Christopher J. Cronin, Zhaoyang Feng and William R. Schafer



Intracellular Ca2+ Imaging in Caehorhabditis elegans

Rex A. Kerr and William R. Schafer



In Vitro Culture of C. elegans Somatic Cells<
Kevin Strange and Rebecca Morrison



Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS Flow Sorting System

Rock Pulak et al.
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