Service-Telefon
+49(0)6151-988520
German English
Warenkorb      SPACERIMGALTERNATEanzeigen
Artikelanzahl:  1
Warenwert: 59,00 €
Versandkosten: 3,95 €
Gesamtbetrag: 62,95 €
Fachgebiete

Details

1891836 Andrew J. Link; Philip Andrews; Joshua LaBaer:
Proteomics
A Cold Spring Harbor Laboratory Course
Preis:   € 59,00

Einband: Paperback
Auflage: 1.Auflage
Verlag: Cold Spring Harbor Laboratory Press
Erscheinungsdatum: 12/2008
Seiten: 200 pp
Abbildungen: Illustrations,appendices,index

ISBN-10: 0-87969-787-3   
ISBN-13: 978-087969787-7


im Warenkorb [1 Exemplar]

zurück
Beschreibung
Based on a popular course at Cold Spring Harbor Laboratory, this new manual assembles cutting–edge protocols, helpful hints, and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using state–of–the–art instrumentation. Detailed protocols involving protein microarrays, liquid chromatography, high–throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI–TOF, and MudPIT are provided, along with well–illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. Proteomics: A Cold Spring Harbor Laboratory Course Manual can be used both as the basis for a course and as a detailed bench manual for those performing indispensable proteomic experiments. It is authored by Andrew J. Link, Philip C. Andrews, and Joshua LaBaer, all leaders in their fields, who bring complementary expertise to the manual.
Inhalt
Preface
Introduction
Highly Recommended Reading
Recommended Web Sites

Experiments
1. Two-dimensional Gel Electrophoresis Analysis of Whole Cell Lysates
2. Purification of Protein Complexes for Mass Spectrometry Analysis
3. Matrix Assisted Laser Desorption Ionization and Time of Flight Mass Spectrometry Analysis of Trypsin-digested Proteins (MALDI-TOF/TOF)
4. High-sensitivity Liquid Chromatography and Tandem Mass Spectrometry Analysis of Protein Complexes (nanoLC-MS/MS)
5. Phosphopeptide Analysis Using IMAC and Mass Spectrometry
6. Multidimensional Protein Identification Technology (MUDPIT) Analysis of Whole Cell Lysates
7. Quantitative Mass Spectrometry Analysis of Whole Cell Extracts (iTRAQ)
8. Analysis and Validation of Tandem Mass Spectra
9. High-throughput Cloning of ORFs: Assembling Large Sets of Expression Constructs
10. Construction of Protein Microarrays Nucleic Acid Programmable Protein Array (NAPPA)

Appendices
Setup of an Electrospray Ionization Source
In-gel Digest
Solution Protein Digest
TCA Precipitation of Proteins
Average and Monoisotopic Masses of Amino Acids
Dipeptide Masses of Amino Acids
Recipes of Solutions
Recipes of Media
Tune File Setup for LTQ
LTQ MS Methods
Offline Desalting
Preparing Competent Cells
DNA Quantification

Zielgruppe/Leser
Proteins and Proteomics
Laboratory Manuals

Autoreninfo
By Andrew J. Link, Vanderbilt University School of Medicine, Nashville, Tennessee; Philip Andrews, University of Michigan Medical School, Ann Arbor; Joshua LaBaer, Harvard University School of Medicine
 © academic-books.com, Alle Rechte vorbehalten.