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542390 Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.:
Short Protocols in Molecular Biology
2 Volume Set
Preis:   € 149,00

Einband: Pb
Auflage: 5. Auflage
Verlag: John Wiley & Sons
Erscheinungsdatum: 12/2002
Seiten: 1512 S.

ISBN-10: 0-471-25092-9   
ISBN-13: 978-0-471-25092-0


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Beschreibung
Recently expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations.
The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research
Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level
Expanded to 2 volumes.
Inhalt
VOLUME 1
Chapter 1: Eschericia Coli,:Plasmids, and Bacteriophages
Chapter 2: Preparation and Analysis of DNA
Chapter 3: Enzymatic Manipulation of DNA AND RNA
Chapter 4: Preparation and Analysis of RNA
Chapter 5: Construction of Recombinant DNA Libraries
Chapter 6: Screening of Recombinant Libraries
Chapter 7: DNA Sequencing
Chapter 8: Mutagenesis of Cloned DNA
Chapter 9: Introduction of DNA into Mammalian Cells
Chapter 10: Analysis of Proteins
Chapter 11: Immunology
VOLUME 2
Chapter 12: DNA-Protein Interactions
Chapter 13: Saccharomyces Cerevisiae
Chapter 14: In Situ Hybridization and Immunohistochemistry
Chapter 15: The Polymerase Chain Reaction
Chapter 16: Protein Expression
Chapter 17: Analysis of Protein Phosphorylation
Chapter 18: Informatics for Molecular Biologists
Chapter 19: Analysis of Protein Interactions
Chapter 20: Chromatin Assembly and Analysis
Chapter 21: Nucleic Acid Arrays
Chapter 22: Generation and Use of Combinatorial Libraries
Chapter 23: Discovery and Analysis of Differentially Expressed Genes in Single Cells and Cell Populations
Appendices

Vorteile
Das Werk wurde speziell dafür konizipiert, dem Leser einen raschen Zugriff auf Schritt-für-Schritt-Anleitungen zu wesentlichen Analysemethoden zu ermöglichen, die in jedem wichtigen Bereich der molekularbiologischen Forschung Anwendung finden.

"Short Protocols in Molecular Biology" ist der maßgebliche und unverzichtbare Laborleitfaden für alle Biowissenschaftler, Forscher und Studenten.

Zielgruppe/Leser
Biowissenschaftler und fortgeschrittene Studenten/Doktoranden

Autoreninfo
Ausubel, F. M. et al. (Hrsg.)
F. M. Ausubel, Massachusetts General Hospital & Harvard Medical School
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