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3021479 Barres, Ben A.; Stevens, Beth:
Purifying and Culturing Neural Cells: A Laboratory Manual
  Preis:   € 59,00

Einband: Paperback
Auflage: 1. Auflage
Verlag: Cold Spring Harbor Laboratory Press New York
Erscheinungsdatum: 11/2013
Seiten: 250 pp. (approx..)
Abbildungen: illus, index

ISBN-10: 1-936113-99-6   
ISBN-13: 978-1-936113-99-6


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Beschreibung
The ability to grow neuronal cells in the laboratory is key to experiments aimed at understanding how the nervous system functions. This book provides detailed descriptions of how cultured neuronal cells can be generated and maintained, covering neurons, astrocytes, vascular cells, and myelinating glia.
Inhalt
Contents

(preliminary)

I. Introduction

II. CNS Neurons
Chapter 1: Purification and Culture of Retinal Ganglion Cells
Alissa Winzeler and Jack T. Wang
Chapter 2: Purification and Culture of Corticospinal Motor Neurons
Wim Mandmakers
Chapter 3: Purification and Culture of Spinal Motor Neurons
David J. Graber and Brent T. Harris
Chapter 4: Purification and Culture of Dorsal Root Ganglion Neurons
J. Bradley Zuchero

III. Astrocytes and Vascular Cells
Chapter 5: Purification and Culture of Astrocytes
Lynette C. Foo
Chapter 6: Purification and Culture of CNS Pericytes
Lu Zhou, Fabien Sohet, and Richard Daneman
Chapter 7: Purification and Culture of CNS Endothelial Cells
Lu Zhou, Fabien Sohet, and Richard Daneman

IV. Myelinating Glia
Chapter 8: Purification and Culture of Oligodendrocyte Lineage Cells
Jason C. Dugas and Ben Emery
Chapter 9: Myelinating Cocultures of Purified Oligodendrocyte Lineage Cells and Retinal Ganglion Cells
Trent A. Watkins and Anja R. Scholze
Chapter 10: Purification of Schwann Cells
Amanda Brosius Lutz
Chapter 11: Designing and Troubleshooting Immunopanning Protocols for Purifying Neural Cells
Ben A. Barres

Index

Zielgruppe/Leser
Cell Biology; Biochemistry; Neurobiology
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