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2054089 Swedlow, Jason; Goldman, Robert; Spector, David:
Live Cell Imaging: A Laboratory Manual, Second Edition
  Preis:   € 89,00

Einband: Paperback
Auflage: 2. Auflage
Verlag: Cold Spring Harbor Laboratory Press
Erscheinungsdatum: 12/2009
Seiten: 650 pp. (approx.)
Abbildungen: illus., appendix, index

ISBN-10: 0-87969-893-4   
ISBN-13: 9780879698935


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Beschreibung
This advanced manual includes evolving methods for studying dynamic changes in living cells and organisms, presenting techniques as well as background material, and serving also as a text in advanced courses. The first section covers principles and fundamental issues of detection and imaging; the second provides detailed protocols for imaging live systems. Unique to this edition are advances in 3-D microscopy: atomic force microscopy and structured illumination microscopy (using OMX). New analytical options are also described: live high-throughput/content screening and computational analysis of live cell data.
Inhalt
Section I: Detection and Approaches to Live Cell Imaging
1. Fluorescent Protein Tracking and Detection
M. Rizzo, M. Davidson, and D. Piston
2. Constructing and Expressing Fluorescent Protein Fusions
D. Spector and B. Goldman
3. Micropatterning CellñSubstrate Adhesions Using Linear Polyacrylamide as the Blocking Agent
W.-h. Guo and Y.-l. Wang
4. CCD Cameras for Fluorescence Imaging of Living Cells
W. Salmon and J. Waters
5. Fluorescence Perturbation Techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP, Photoactivation, Photoconversion, and FLIP
A. Bancaud, S. Huet, G. Rabut, and J. Ellenberg
6. Imaging Protein State in Cells
H. Grecco and P. Bastiaens
7. A Versatile, Multicolor Total Internal Reflection Fluorescence and Spinning-Disk Confocal Microscope System for High-Resolution Live Cell Imaging
W. Shin, R. Fischer, P. Kanchanawong, Y. Kim, J. Lim, K. Myers, Y. Nishimura, S. Plotnikov, I. Thievessen, D. Yarar, B. Sabass, and C. Waterman
8. Confocal Microscopy, Deconvolution, and Structured Illumination Methods
J. Murray
9. Atomic-Force Microscopy for Biological Imaging and Mechanical Testing across Length Scales
M. Plodinec, M. Loparic, and U. Aebi
10. OMX: A New Platform for Multimodal, Multichannel Wide-Field Imaging
I. Dobbie, E. King, R.M. Parton, P. Carlton, J.W. Sedat, J.R. Swedlow, and I. Davis
11. Digital Scanned Laser Light-Sheet Fluorescence Microscopy
P.J. Keller and E.H.K. Stelzer
12. First Step for Fluorescence Correlation Spectroscopy for the Live Cell
M. Kinjo, H. Sakata, and S. Mikuni
13. Tracking and Quantitative Analysis of Dynamic Movements of Cells and Particles
K. Rohr, W. Godinez, N. Harder, S. Wörz, J. Mattes, W. Tvaruskó, and R. Eils
14. Imaging Techniques for Measuring Cell Materials Properties
K. Kasza, D. Vader, S. Köster, N. Wang, and D. Weitz
15. Computational Image Analysis of Cellular Dynamics: A Case Study Based on Particle Tracking
K. Jaqaman and G. Danuser
16. Software Tools, Data Structures, and Interfaces for Microscope Imaging
N. Stuurman and J.R. Swedlow
17. High-Throughput Microscopy Using Live Mammalian Cells
S. Terjung, T. Walter, A. Seitz, B. Neumann, R. Pepperkok, and J. Ellenberg

Section II: Imaging of Live Cells and Organisms
18. In Vivo Imaging of Mammalian Cells
J. Swedlow, I. Porter, M. Posch, and S. Swift
19. Live Cell Imaging of Yeast
D. Rines, D. Thomann, J. Dorn, P. Goodwin, and P. Sorger
20. Live Imaging of Caenorhabditis elegans
B. Podbilewicz and Y. Gruenbaum
21. Live Cell Imaging of Plants
Y. Fang and D. Spector
22. Pushing the Limits of Live Cell Imaging in Drosophila
R. Parton, A.M. Vallés, I. Dobbie, and I. Davis
23. Dynamic, Long-Term, In Vivo Imaging of Tumor-Stroma Interactions in Mouse Models of Breast Cancer Using Spinning Disk Confocal Microscopy
A.J. Ewald, Z. Werb, and M. Egeblad
24. High-Resolution Multiphoton Imaging of Tumors In Vivo
J. Wyckoff, B. Gligorijevic, D. Entenberg, J. Segall, and J. Condeelis
25. Correlated Live Cell Light and Electron Microscopy Using Tetracysteine Tags and Biarsenicals
G.M. Gaietta, T.J. Deerinck, and M.H. Ellisman
26. Intravital Microscopy of Normal and Diseased Tissues in the Mouse
R.K. Jain, L.L. Munn, and D. Fukumura
27. Imaging Lipids in Living Cells
C. Schultz, A.B. Neef, T.W. Gadella, Jr., and J. Goedhart
28. Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes
Y.G. Strukov, M. Plutz, and A.S. Belmont
29. Imaging Gene Expression in Living Cells
S. Janicki and D. Spector
30. Studying Mitosis in Cultured Mammalian Cells
P. Wadsworth
31. Imaging Intermediate Filament Proteins in Living Cells
E.R. Kuczmarski, T. Shimi, and R.D. Goldman
32. Methods for Expressing and Analyzing GFP-Tubulin and GFP-Microtubule-Associated-Proteins
H.V. Gooodson, J.S. Dzurisin, and P. Wadsworth
33. Imaging of Membrane Systems and Membrane Traffic in Living Cells
E.L. Snapp and P. Lajoie
34. Imaging Live Cells under Mechanical Stress
B.P. Helmke and P.F. Davies
35. Imaging Single Molecules Using Total Internal Reflection Microscopy
S. Reck-Peterson, N. Derr, and N. Stuurman
36. Cellular Imaging Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
D. Toomre
37. Visualization and Quantification of Single RNA Molecules in Living Cells
Y. Shav-Tal, S.M. Shenoy, and R.H. Singer

Vorteile
Many laboratories that are engaged in the examination of
fixed specimens tend to be outfitted with technologically
advanced microscopes. As we begin to push the limits of
our detection methods, the challenge is now to implement
these extraordinary technologies to the living cell or organism.
It is for this reason that the latest edition of Live Cell
Imaging comes as a welcome addition to the cell biologist’s
bookshelf. This book encompasses all of the information
needed to approach a live cell imaging problem, including
detailed descriptions of the instrumentation, genetic tools,
and analytical techniques currently available in the field.
These protocols are complemented by the second part of
the book, which covers specific applications for live imaging
in a wide variety of established model systems.
There are many features of this book that make it a
useful resource for both the beginning and advanced user.
First, the book is composed of 37 self-contained chapters,
each written by leading authorities on these subjects. Thus,
the user who seeks to purchase new equipment or select
the appropriate fluorophore will find it useful to peruse
chapters from the first part of the book, such as the chapters
on charge-coupled device cameras or fluorescent protein
tracking. On the other hand, users who seek to implement
the imaging technologies already available to them can
delve directly into the second part of the book, which deals
with specific examples of live imaging certain cell types,
tissues, or components. Second, many of the chapters are
accompanied by superb movies, which are conveniently
indexed at the beginning of the book, and also clearly
displayed on the book’s website in obvious drop-down
boxes ~www.chsprotocols.org/livecellimaging!. Third, the majority
of chapters contain relevant links to further resources,
allowing the user to have access to sites with continuing
education or tutorials ~such as many microscope manufacturer
websites! and access to necessary analytical tools,
including both proprietary and open source software. Finally,
the protocols are detailed and easy to follow, with
clearly outlined pitfalls and troubleshooting strategies for
problems commonly encountered during live imaging,
such as phototoxicity or focal drift. Many of these protocols
also include comprehensive tables that list currently available
constructs, dyes, or reagents and their relevant references.
For example, there are some tables that list all of
the organelle markers available for a particular model
organism such as the worm or fruitfly, and there are other
tables that span all the model systems to list the available
resources for studying a particular cellular component, such
as microtubules.
In addition to the many practical considerations that
make Live Cell Imaging a useful resource, this book also
provides an authoritative overview of the vast potential that
live imaging strategies have for addressing previously unapproachable
biological problems. The selection of material
that is covered by the book captures the excitement of the
most recent advances in microscopy and quantitative analysis.
These include, but are not limited to, comprehensive
chapters on super-resolution light microscopes ~such as the
structured illumination-based OMX microscope, or the
photoactivatable/photoswitchable fluorophore-based PALM/
STORM microscopes!, thick-tissue imaging ~using multiphoton
or light sheet-based fluorescence microscopes!, protein
state dynamics ~using fluorescence recovery after photobleaching,
fluorescence resonance energy transfer, and other
fluorescence-based methods!, single particle analysis ~using
four-dimensional tracking and computational models!, and
high-throughput screening. All of these techniques are presented
within the context of interesting biological problems,
such as the live imaging of mouse tumors or the resolution
of cellular ultrastructure.
In conclusion, I highly recommend this latest edition of
Live Cell Imaging to anybody who wishes to implement
available technologies to probe the in vivo dynamics of a
protein or tissue of interest. This book allows the reader to
contemplate how to use existing equipment with some
minor adjustments or simple application of reagents and
analytical tools. Furthermore, this book provides a comprehensive
analysis of the latest imaging technologies available
in the field and should therefore be invaluable to any
researcher who wishes to find a discussion of all of these
techniques and applications under one umbrella.
Zehra F. Nizami
Graduate Student
The Johns Hopkins University
Baltimore, MD and
The Carnegie Institution of Washington
Washington, D.C.
MICROSCOPY AND MICROANALYSIS VOL 16 / Issue 5

Autoreninfo
Edited By Robert D. Goldman, Feinberg School of Medicine Northwestern University; Jason R. Swedlow, The University of Dundee; David L. Spector, Cold Spring Harbor Laboratory
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