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1720571 Coligan, John E.; Dunn, Ben M.; Speicher, David W.:
Short Protocols in Protein Science
  Preis:   € 105,00

Einband: Pb
Auflage: 1. Auflage
Verlag: John Wiley & Sons
Erscheinungsdatum: 11/2003
Seiten: 810 S.

ISBN-10: 0-471-48338-9   
ISBN-13: 978-0-471-48338-0


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Beschreibung
Short Protocols in Protein Science provides condensed descriptions of more than 500 protocols compiled from Current Protocols in Protein Science. Drawing from both the original "core" manual as well as the quarterly update service, this compendium includes all step-by-step descriptions of the principal methods covered in Current Protocols in Protein Science.
Inhalt
Strategies of Protein Purification & Characterization.
Computational Analysis.
Detection and Assay Methods.
Extraction, Stabilization, and Concentration.
Production of Recombinant Proteins.
Purification of Recombinant Proteins.
Characterization of Recombinant Proteins.
Conventional Chromatographic Separations.
Affinity Purification.
Electrophoresis-Updates Expected for CPPS 10.1, 10.3, 10.10 & 10.11.
Chemical Analysis.
Post-Translational Modification: Glycosylation.
Post-Translational Modification: Phosphorylation and Phosphatases.
Post-Translational Modification: Specialized Applications.
Chemical Modification of Proteins.
Mass Spectrometry.
Preparation and Handling of Peptides.
Identification of Protein Interactions.
Quantitation of Protein Interactions.
Peptidases.
Appendix 1: Reagents and Solutions.
Appendix 2: Useful Measurements.
Appendix 3: Commonly Used Techinques.
Appendix 4: Suppliers.
References.

Zielgruppe/Leser
Biowissenschaftler

Autoreninfo
Dunn, B. M. / Speicher, D. W. / Wingfield, P. T.
B. M. Dunn, University of Florida, Gainesville, Fla.; D. W. Speicher, The Wistar Institute, Philadelphia, Penn.; P. T. Wingfield, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Md
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