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2183270 Dahmann, Christian:
Drosophila
Methods and Protocols
Preis:   € 139,09

Band: 420, Einband: Pb
Auflage: 1st Edition. Softcover version of original hardcover edition 2008
Verlag: Humana Press
Erscheinungsdatum: 10/2010
Seiten: 437 S.

ISBN-10: 1-61737-791-0   
ISBN-13: 978-1-61737-791-4


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Beschreibung
Drosophila is a comprehensive collection of methods and protocols for Drosophila, one of the oldest and most commonly used model organisms in modern biology. The protocols are written by the scientists who invented the methods. The text presents a diverse set of techniques that range from the basic handling of flies to more complex applications. This is the perfect reference manual for Drosophila researchers.
Inhalt
Methods in Molecular Biology Series Editor: John M. Walker
Volume: Drosophila: Methods and Protocols
Edited by Christian Dahmann, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Contents
Preface
Contributors
1 Drosophila melanogaster and the Development of Biology in the XX century
Alfonso Martinez Arias
2 Getting started: An overview on raising and using Drosophila
Hugo Stocker and Peter Gallant
3 FlyBase: A database for the Drosophila research community
Rachel Drysdale
4 The use of P-element transposons to generate transgenic flies
André Bachmann and Elisabeth Knust
5 The GAL4 system: a versatile system for the expression of genes
David A. Elliott and Andrea H. Brand
6 P-element mutagenesis
Thomas Hummel and Christian Klämbt
7 EMS Screens: From mutagenesis to screening and mapping
Christian Bökel
8 RNAi screening in cultured Drosophila cells
Sandra Steinbrink and Michael Boutros
9 Methods for homologous recombination in Drosophila
Keith A. Maggert, Wei J Gong, and Kent G. Golic
10 Recombinases and their use in gene activation, gene inactivation, and transgenesis
Johannes Bischof and Konrad Basler
11 Cuticle preparation of Drosophila embryos and larvae
Cyrille Alexandre
12 Immunolabelling of embryos
H.- Arno J. Müller
13 Imaging cellular and molecular dynamics in live embryos using fluorescent proteins
Matthieu Cavey and Thomas Lecuit
14 Analysis of neuromuscular junctions: histology and in vivo imaging
Andreas Schmid and Stephan J. Sigrist
15 Immunolabelling of imaginal discs
Thomas Klein
16 Imaging Drosophila pupal wing morphogenesis
Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande, and Suzanne Eaton
17 Microscopic analysis of the adult Drosophila retina using semi thin plastic sections
Konstantin Gaengel and Marek Mlodzik
18 Fluorescent in situ hybridization protocols in Drosophila embryos and tissues
Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause
19 Linear RNA amplification for the production of microarray hybridization probes
Ansgar Klebes and Thomas B. Kornberg
20 An overview of the identification, detection, and functional analysis of Drosophila microRNAs
Nicholas Sokol
21 Extraction and immunoblotting of proteins from embryos
Andreas Wodarz
22 Purification of Drosophila protein complexes for mass spectrometry
Christoph Jüschke and Jürgen A. Knoblich
23 Mass production of Drosophila embryos and chromatographic purification of native protein complexes
Natascha Kunert and Alexander Brehm
24 Flow cytometric analysis of Drosophila cells
Aida Flor A. de la Cruz and Bruce A. Edgar
25 Drosophila cell lines as model systems and as an experimental tool
Buzz Baum and Lucy Cherbas
Index
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