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2054087 McGarry, Michael P.; Protheroe, Cheryl A.; Lee, James J.:
Mouse Hematology: A Laboratory Manual
  Preis:   € 66,00

Einband: Paperback
Auflage: 1. Auflage
Verlag: Cold Spring Harbor Laboratory Press
Erscheinungsdatum: 12/2009
Seiten: 90 pp. (approx.)
Abbildungen: illus., appendix, index;

ISBN-10: 0-87969-886-1   
ISBN-13: 9780879698867


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Beschreibung
The mouse has become a standard laboratory model organism, particularly for the study of hematopoiesis, the immune system, and inflammation. Although laboratories studying stem cells, blood, and blood-forming tissues have assimilated many new molecular diagnostic methods, the identification of cell lineages through classical light microscopic techniques is often poorly understood and practiced. Mouse Hematology presents a concise review of conventional methods for the preparation, enumeration, and microscopic examination of blood and blood-forming tissues of the laboratory mouse. Along with a short laboratory manual featuring detailed protocols, Mouse Hematology includes a DVD of short video demonstrations of the techniques and a poster of blood cell types for easy identification at the microscope. These rapid, inexpensive assessments can save valuable time and resources essential to the design, development, and interpretation of experiments.
Inhalt
Contents
(preliminary)

Preface
1. Collection of Peripheral Blood
2. Counting Red Blood Cells, Platelets, and Viable Nucleated White Blood Cells
3. Peripheral Blood Films and Cytospin Preparations
4. Cell Differential Assessments of Peripheral Blood Films
5. Preparation of Bone Marrow and Other Lymphohematopoietic Tissues for Microscopic Examination
6. Cell Differential Assessments of Bone Marrow and Other Hematopoietic Compartments
Conclusion
Appendix
Index

Autoreninfo
Michael P. McGarry, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona; Cheryl A. Protheroe, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona; James J. Lee, Department of Biochemistry and Molecular Biology, Mayo Clinic Arizona
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